AP08741PU-N

antibody from Acris Antibodies GmbH

Targeting: SYN1 MRX50

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP08741PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP08741PU-N, RRID:AB_1975712

Product name

anti Synapsin-1 pSer9

Antibody type

Polyclonal

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser9 of Synapsin I.

Reactivity

Human, Mouse, Rat, Bovine, Xenopus, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

References

Submitted references

Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant.

Shimomura S, Nagamine T, Hatano N, Sueyoshi N, Kameshita I
Journal of biochemistry 2010 May;147(5):711-22

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot of rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser9 synapsin I antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9