Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- MAB248-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BDNF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross-reactivity with recombinant human (rh) beta-NGF and rr beta-NGF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P23560
- Isotype
- IgG
- Antibody clone number
- 35928
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival.
Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish.
Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after post-natal nicotine and intermittent hypercapnic hypoxia.
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of Intermittent Hypercapnic Hypoxia.
Vanhecke E, Adriaenssens E, Verbeke S, Meignan S, Germain E, Berteaux N, Nurcombe V, Le Bourhis X, Hondermarck H
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1741-52
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1741-52
Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish.
Diekmann H, Anichtchik O, Fleming A, Futter M, Goldsmith P, Roach A, Rubinsztein DC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 4;29(5):1343-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 4;29(5):1343-9
Brain-derived neurotrophic factor (BDNF) and TrkB in the piglet brainstem after post-natal nicotine and intermittent hypercapnic hypoxia.
Tang S, Machaalani R, Waters KA
Brain research 2008 Sep 26;1232:195-205
Brain research 2008 Sep 26;1232:195-205
Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
Samah B, Porcheray F, Gras G
Clinical and experimental immunology 2008 Mar;151(3):476-86
Clinical and experimental immunology 2008 Mar;151(3):476-86
BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
Journal of neuroimmunology 2008 Jul 15;197(2):128-39
Journal of neuroimmunology 2008 Jul 15;197(2):128-39
Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
Cancer research 2008 Dec 1;68(23):9996-10003
Cancer research 2008 Dec 1;68(23):9996-10003
Brain-derived neurotrophic factor mRNA and protein in the piglet brainstem and effects of Intermittent Hypercapnic Hypoxia.
Peiris TS, Machaalani R, Waters KA
Brain research 2004 Dec 10;1029(1):11-23
Brain research 2004 Dec 10;1029(1):11-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Human BDNF Monoclonal Antibody (Catalog # MAB248) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.