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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 209-401-C27 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#209-401-C27, RRID:AB_10707147
- Product name
- Anti-BDNF (RABBIT) Antibody - 209-401-C27
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Anti-human BDNF antibody in western blot shows detection of recombinant human BDNF raised in E.coli. Recombinant truncated (0.1 µg, 27 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Rockland's, Inc. Anti-Human BDNF. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody (611-143-122) diluted 1:20,000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
- Validation comment
- Western Blot
