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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PA1-18357 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BDNF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A pain killer without analgesic tolerance designed by co-targeting PSD-95-nNOS interaction and α2-containning GABA(A)Rs.
Prolonged Use of NMDAR Antagonist Develops Analgesic Tolerance in Neuropathic Pain via Nitric Oxide Reduction-Induced GABAergic Disinhibition.
Li J, Zhang L, Xu C, Shen YY, Lin YH, Zhang Y, Wu HY, Chang L, Zhang YD, Chen R, Zhang ZP, Luo CX, Li F, Zhu DY
Theranostics 2021;11(12):5970-5985
Theranostics 2021;11(12):5970-5985
Prolonged Use of NMDAR Antagonist Develops Analgesic Tolerance in Neuropathic Pain via Nitric Oxide Reduction-Induced GABAergic Disinhibition.
Li J, Zhang L, Xu C, Lin YH, Zhang Y, Wu HY, Chang L, Zhang YD, Luo CX, Li F, Zhu DY
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2020 Jul;17(3):1016-1030
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2020 Jul;17(3):1016-1030
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-BDNF Polyclonal Antibody (Product # PA1-18357) and a 34kDa band corresponding to Brain-derived neurotrophic factor was observed across the panel tested except for Mouse liver and spleen which are reported to be negative. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Liver (Lane 2), Mouse Spleen (Lane 3) and Rat Brain (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). An uncharacterized band (*) at ~40kDa was also observed in mouse brain.
