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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB31751 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Pro-BDNF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Pro-BDNF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human beta-NGF, NT-4, Pro-NT-3 or Pro-NT-4 is observed. In Western blots, 100% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human mature BDNF or recombinant human NT-3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P23560- Isotype
- IgG
- Antibody clone number
- 584412
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Pro-BDNF by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Pro-BDNF Monoclonal Antibody (Catalog # MAB31751) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pro-BDNF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
