Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB100-98682 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-98682, RRID:AB_1290643
- Product name
- Rabbit Polyclonal BDNF Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Specific for mature BDNF.
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Calpain inhibition ameliorates depression-like behaviors by reducing inflammation and promoting synaptic protein expression in the hippocampus.
Electroacupuncture Therapy Ameliorates Motor Dysfunction via Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor in a Mouse Model of Parkinson's Disease.
BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis.
Neuroprotective Effects of Doxycycline in the R6/2 Mouse Model of Huntington's Disease.
Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression.
Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model.
Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia.
Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke.
Immunofluorescence/fluorescence assessment of brain-derived neurotrophic factor, c-Fos activation, and apoptosis in the brain of zebrafish (Danio rerio) larvae exposed to glufosinate.
Differential Effects of Low- and High-dose Zinc Supplementation on Synaptic Plasticity and Neurogenesis in the Hippocampus of Control and High-fat Diet-fed Mice.
Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.
Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice.
Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory.
Song Z, Shen F, Zhang Z, Wu S, Zhu G
Neuropharmacology 2020 Sep 1;174:108175
Neuropharmacology 2020 Sep 1;174:108175
Electroacupuncture Therapy Ameliorates Motor Dysfunction via Brain-Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor in a Mouse Model of Parkinson's Disease.
Pak ME, Ahn SM, Jung DH, Lee HJ, Ha KT, Shin HK, Choi BT
The journals of gerontology. Series A, Biological sciences and medical sciences 2020 Mar 9;75(4):712-721
The journals of gerontology. Series A, Biological sciences and medical sciences 2020 Mar 9;75(4):712-721
BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis.
Ding H, Chen J, Su M, Lin Z, Zhan H, Yang F, Li W, Xie J, Huang Y, Liu X, Liu B, Zhou X
Journal of neuroinflammation 2020 Jan 13;17(1):19
Journal of neuroinflammation 2020 Jan 13;17(1):19
Neuroprotective Effects of Doxycycline in the R6/2 Mouse Model of Huntington's Disease.
Paldino E, Balducci C, La Vitola P, Artioli L, D'Angelo V, Giampà C, Artuso V, Forloni G, Fusco FR
Molecular neurobiology 2020 Apr;57(4):1889-1903
Molecular neurobiology 2020 Apr;57(4):1889-1903
Antidepressant-Like Effects of Gyejibokryeong-hwan in a Mouse Model of Reserpine-Induced Depression.
Park BK, Kim YR, Kim YH, Yang C, Seo CS, Jung IC, Jang IS, Kim SH, Lee MY
BioMed research international 2018;2018:5845491
BioMed research international 2018;2018:5845491
Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model.
Tang Y, Liu L, Xu D, Zhang W, Zhang Y, Zhou J, Huang W
Brain, behavior, and immunity 2018 Feb;68:248-260
Brain, behavior, and immunity 2018 Feb;68:248-260
Combined therapy involving electroacupuncture and treadmill exercise attenuates demyelination in the corpus callosum by stimulating oligodendrogenesis in a rat model of neonatal hypoxia-ischemia.
Pak ME, Jung DH, Lee HJ, Shin MJ, Kim SY, Shin YB, Yun YJ, Shin HK, Choi BT
Experimental neurology 2018 Feb;300:222-231
Experimental neurology 2018 Feb;300:222-231
Potential benefits of mesenchymal stem cells and electroacupuncture on the trophic factors associated with neurogenesis in mice with ischemic stroke.
Kim YR, Ahn SM, Pak ME, Lee HJ, Jung DH, Shin YI, Shin HK, Choi BT
Scientific reports 2018 Feb 1;8(1):2044
Scientific reports 2018 Feb 1;8(1):2044
Immunofluorescence/fluorescence assessment of brain-derived neurotrophic factor, c-Fos activation, and apoptosis in the brain of zebrafish (Danio rerio) larvae exposed to glufosinate.
Çomakli S, Köktürk M, Topal A, Özkaraca M, Ceyhun SB
Neurotoxicology 2018 Dec;69:60-67
Neurotoxicology 2018 Dec;69:60-67
Differential Effects of Low- and High-dose Zinc Supplementation on Synaptic Plasticity and Neurogenesis in the Hippocampus of Control and High-fat Diet-fed Mice.
Nam SM, Kim JW, Kwon HJ, Yoo DY, Jung HY, Kim DW, Hwang IK, Seong JK, Yoon YS
Neurochemical research 2017 Nov;42(11):3149-3159
Neurochemical research 2017 Nov;42(11):3149-3159
Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.
Atasoy İL, Dursun E, Gezen-Ak D, Metin-Armağan D, Öztürk M, Yılmazer S
Journal of chemical neuroanatomy 2017 Mar;80:19-26
Journal of chemical neuroanatomy 2017 Mar;80:19-26
Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice.
Lee HI, Lee SW, Kim NG, Park KJ, Choi BT, Shin YI, Shin HK
Journal of biophotonics 2017 Dec;10(12):1761-1771
Journal of biophotonics 2017 Dec;10(12):1761-1771
Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory.
Efstathopoulos P, Kourgiantaki A, Karali K, Sidiropoulou K, Margioris AN, Gravanis A, Charalampopoulos I
Translational psychiatry 2015 Nov 24;5:e685
Translational psychiatry 2015 Nov 24;5:e685
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BDNF Antibody [NB100-98682] - Human platelet lysate and recombinant mature BDNF. Blocking: 1% LFDM for 30 min at RT; primary antibody (1:1000) incubated at 4C overnight.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BDNF Antibody [NB100-98682] - Mouse hippocampus. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.