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- Antibody Data
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- References [4]
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- Western blot [7]
- Immunocytochemistry [1]
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- Product number
- GTX114075 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114075, RRID:AB_10619535
- Product name
- GCLM antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2.
Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317
Free radical biology & medicine 2018 Feb 1;115:309-317
A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Choi EJ, Jung BJ, Lee SH, Yoo HS, Shin EA, Ko HJ, Chang S, Kim SY, Jeon SM
Oncogene 2017 Sep 14;36(37):5285-5295
Oncogene 2017 Sep 14;36(37):5285-5295
Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2.
Furuya AK, Sharifi HJ, Jellinger RM, Cristofano P, Shi B, de Noronha CM
PLoS pathogens 2016 Apr;12(4):e1005581
PLoS pathogens 2016 Apr;12(4):e1005581
Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Liu XM, Peyton KJ, Durante W
American journal of physiology. Heart and circulatory physiology 2013 Jun 15;304(12):H1634-43
American journal of physiology. Heart and circulatory physiology 2013 Jun 15;304(12):H1634-43
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: C8D30 12% SDS PAGE GTX114075 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: rat liver 12% SDS PAGE GTX114075 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE GCLM antibody GTX114075 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody (GTX114075) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using GCLM(GTX114075) antibody at 1:200 dilution.
