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- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN952478 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glutamate-Cysteine Ligase, Modifier Subunit (GCLM) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references Glutathione pathway genetic polymorphisms and lung cancer survival after platinum-based chemotherapy.
The mutagenicity of nitrosamides in Salmonella typhimurium.
Moyer AM, Sun Z, Batzler AJ, Li L, Schaid DJ, Yang P, Weinshilboum RM
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2010 Mar;19(3):811-21
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2010 Mar;19(3):811-21
The mutagenicity of nitrosamides in Salmonella typhimurium.
Lijinsky W, Andrews AW
Mutation research 1979 Sep;68(1):1-8
Mutation research 1979 Sep;68(1):1-8
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of GCLM (arrow) using GCLM / GLCLR Antibody (C-term) Cat.-No AP51801PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCLM gene.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of GCLM / GLCLR Antibody (C-term) Cat.-No AP51801PU-N in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GCLM / GLCLR Antibody (C-term) Cat.-No AP51801PU-N with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded skeletal muscle reacted with GCLM / GLCLR Antibody (C-term) Cat.-No AP51801PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using GCLM / GLCLR Antibody (C-term) Cat.-No AP51801PU-N(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
