H00005268-M01

antibody from Abnova Corporation

Targeting: SERPINB5 maspin, PI5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005268-M01

Provider

Abnova Corporation

Product name

SERPINB5 monoclonal antibody (M01), clone 1F7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant SERPINB5.

Antigen sequence

MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICL
STSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGF
QTVTSDVNKPSSFYSLKLIKRLYVDKSLNLSTEFI
SSTKRPYAKELETVDFKDKLEETKGQINNSIKDLT
DGHFENILADNSVNDQTKILVVNAAYFVGKWMKKF
PESETKECPFRVNRVCGAACSSKRSPIIDVKNDRD
RVGHKSIPMRNLRARPAKCLS


Isotype

IgG

Antibody clone number

1F7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SERPINB5 monoclonal antibody (M01), clone 1F7. Western Blot analysis of SERPINB5 expression in human pancreas.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SERPINB5 expression in transfected 293T cell line by SERPINB5 monoclonal antibody (M01), clone 1F7.Lane 1: SERPINB5 transfected lysate (Predicted MW: 25.52 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SERPINB5 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SERPINB5 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol