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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB8227 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8227, RRID:AB_10615322
- Product name
- SERPINB5 monoclonal antibody, clone AT2N6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant SERPINB5.
- Isotype
- IgG
- Antibody clone number
- AT2N6
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tumor suppressive maspin and epithelial homeostasis.
Maspin: the new frontier.
Lockett J, Yin S, Li X, Meng Y, Sheng S
Journal of cellular biochemistry 2006 Mar 1;97(4):651-60
Journal of cellular biochemistry 2006 Mar 1;97(4):651-60
Maspin: the new frontier.
Khalkhali-Ellis Z
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Dec 15;12(24):7279-83
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Dec 15;12(24):7279-83
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis from cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with SERPINB5 monoclonal antibody, clone AT2N6 (Cat # MAB8227) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with PI (Red) and SERPINB5 monoclonal antibody, clone AT2N6 (Cat # MAB8227) (1 : 500) with Alexa 488 (Green).
- Validation comment
- Immunofluorescence
