Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN954746 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5) (AA 101-131), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 101-131 amino acids from the Central region of human SERPINB5 / Maspin
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 101-131,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Aberrant maspin expression is involved in early carcinogenesis of gallbladder cancer.
Ezrin, maspin, peroxiredoxin 2, and heat shock protein 27: potential targets of a streptococcal-induced autoimmune response in psoriasis.
Clinicopathological correlates and prognostic significance of maspin expression in 450 patients after potentially curative resection of node-positive colonic cancer.
Maspin modulates adhesion of bladder carcinoma cells to vascular endothelium.
Kim J, Jang KT, Kim KH, Park JW, Chang BJ, Lee KH, Lee JK, Heo JS, Choi SH, Choi DW, Rhee JC, Lee KT
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2010 Oct;31(5):471-6
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2010 Oct;31(5):471-6
Ezrin, maspin, peroxiredoxin 2, and heat shock protein 27: potential targets of a streptococcal-induced autoimmune response in psoriasis.
Besgen P, Trommler P, Vollmer S, Prinz JC
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5392-402
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5392-402
Clinicopathological correlates and prognostic significance of maspin expression in 450 patients after potentially curative resection of node-positive colonic cancer.
Fung CL, Chan C, Jankova L, Dent OF, Robertson G, Molloy M, Bokey L, Chapuis PH, Lin BP, Clarke SJ
Histopathology 2010 Feb;56(3):319-30
Histopathology 2010 Feb;56(3):319-30
Maspin modulates adhesion of bladder carcinoma cells to vascular endothelium.
Juengel E, Beecken WD, Mundiyanapurath S, Engl T, Jonas D, Blaheta RA
World journal of urology 2010 Aug;28(4):465-71
World journal of urology 2010 Aug;28(4):465-71
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINB5 / Maspin Antibody (Center) Cat.-No AP52612PU-N in A2058 cell line lysates (35ug/lane). This demonstrates the Maspin antibody detected the Maspin protein (arrow).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- western blot analysis of SERPINB5 / Maspin Antibody (Center) Cat.-No AP52612PU-N in mouse bladder tissue lysates (35ug/lane). This demonstrates the Maspin antibody detected the Maspin protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SERPINB5 / Maspin Antibody (Center) Cat.-No AP52612PU-N with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue reacted with SERPINB5 / Maspin Antibody (Center) Cat.-No AP52612PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.