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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX70137 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70137, RRID:AB_369386
- Product name
- CENPF antibody [14C10 1D8]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Does protein expression predict recurrence of benign World Health Organization grade I meningioma?
M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis.
Mitosin, a novel marker of cell proliferation and early recurrence in intracranial meningiomas.
Localization of human SMC1 protein at kinetochores.
Molecular markers of endometrial epithelial cell mitogenesis mediated by the Sp/Krüppel-like factor BTEB1.
Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis.
Kärjä V, Sandell PJ, Kauppinen T, Alafuzoff I
Human pathology 2010 Feb;41(2):199-207
Human pathology 2010 Feb;41(2):199-207
M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis.
Abaza A, Soleilhac JM, Westendorf J, Piel M, Crevel I, Roux A, Pirollet F
The Journal of biological chemistry 2003 Jul 25;278(30):27844-52
The Journal of biological chemistry 2003 Jul 25;278(30):27844-52
Mitosin, a novel marker of cell proliferation and early recurrence in intracranial meningiomas.
Konstantinidou AE, Korkolopoulou P, Kavantzas N, Mahera H, Thymara I, Kotsiakis X, Perdiki M, Patsouris E, Davaris P
Histology and histopathology 2003 Jan;18(1):67-74
Histology and histopathology 2003 Jan;18(1):67-74
Localization of human SMC1 protein at kinetochores.
Gregson HC, Van Hooser AA, Ball AR Jr, Brinkley BR, Yokomori K
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2002;10(4):267-77
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2002;10(4):267-77
Molecular markers of endometrial epithelial cell mitogenesis mediated by the Sp/Krüppel-like factor BTEB1.
Simmen RC, Zhang XL, Michel FJ, Min SH, Zhao G, Simmen FA
DNA and cell biology 2002 Feb;21(2):115-28
DNA and cell biology 2002 Feb;21(2):115-28
Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis.
Sorensen CS, Lukas C, Kramer ER, Peters JM, Bartek J, Lukas J
Molecular and cellular biology 2000 Oct;20(20):7613-23
Molecular and cellular biology 2000 Oct;20(20):7613-23
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.A. 30 ?g HeLa whole cell lysate/extract (untreated) B. 30 ?g HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)5% SDS-PAGECENPF antibody [14C10 1D8] (GTX70137) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:200.
