NB500-101

Rodriguez-Rodriguez JA, Lewis C, McKinley KL, Sikirzhytski V, Corona J, Maciejowski J, Khodjakov A, Cheeseman IM, Jallepalli PV
Current biology : CB 2018 Nov 5;28(21):3422-3429.e5

The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases.

Ciossani G, Overlack K, Petrovic A, Huis In 't Veld PJ, Koerner C, Wohlgemuth S, Maffini S, Musacchio A
The Journal of biological chemistry 2018 Jun 29;293(26):10084-10101

Microtubules assemble near most kinetochores during early prometaphase in human cells.

Sikirzhytski V, Renda F, Tikhonenko I, Magidson V, McEwen BF, Khodjakov A
The Journal of cell biology 2018 Aug 6;217(8):2647-2659

Deregulation of the spindle assembly checkpoint is associated with paclitaxel resistance in ovarian cancer.

Chong T, Sarac A, Yao CQ, Liao L, Lyttle N, Boutros PC, Bartlett JMS, Spears M
Journal of ovarian research 2018 Apr 4;11(1):27

Regulation of pairing between broken DNA-containing chromatin regions by Ku80, DNA-PKcs, ATM, and 53BP1.

Yamauchi M, Shibata A, Suzuki K, Suzuki M, Niimi A, Kondo H, Miura M, Hirakawa M, Tsujita K, Yamashita S, Matsuda N
Scientific reports 2017 Feb 3;7:41812

Unattached kinetochores rather than intrakinetochore tension arrest mitosis in taxol-treated cells.

Magidson V, He J, Ault JG, O'Connell CB, Yang N, Tikhonenko I, McEwen BF, Sui H, Khodjakov A
The Journal of cell biology 2016 Feb 1;212(3):307-19

CaMKKβ-AMPKα2 signaling contributes to mitotic Golgi fragmentation and the G2/M transition in mammalian cells.

Lee IJ, Lee CW, Lee JH
Cell cycle (Georgetown, Tex.) 2015;14(4):598-611

Pre-exposure to ionizing radiation stimulates DNA double strand break end resection, promoting the use of homologous recombination repair.

Nakajima NI, Hagiwara Y, Oike T, Okayasu R, Murakami T, Nakano T, Shibata A
PloS one 2015;10(3):e0122582

MicroRNA-205 inhibits cancer cell migration and invasion via modulation of centromere protein F regulating pathways in prostate cancer.

Nishikawa R, Goto Y, Kurozumi A, Matsushita R, Enokida H, Kojima S, Naya Y, Nakagawa M, Ichikawa T, Seki N
International journal of urology : official journal of the Japanese Urological Association 2015 Sep;22(9):867-77

Adaptive changes in the kinetochore architecture facilitate proper spindle assembly.

Magidson V, Paul R, Yang N, Ault JG, O'Connell CB, Tikhonenko I, McEwen BF, Mogilner A, Khodjakov A
Nature cell biology 2015 Sep;17(9):1134-44

Unusual prolongation of radiation-induced G2 arrest in tumor xenografts derived from HeLa cells.

Kaida A, Miura M
Cancer science 2015 Oct;106(10):1370-6

Lamins position the nuclear pores and centrosomes by modulating dynein.

Guo Y, Zheng Y
Molecular biology of the cell 2015 Oct 1;26(19):3379-89

Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.

Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, Brako L, Garcia-Barrio M, Ye M, Wei X, Zou H, Ding X, Liu L, Liu X, Yao X
The Journal of biological chemistry 2015 Nov 6;290(45):27053-66

A nonsense mutation of human XRCC4 is associated with adult-onset progressive encephalocardiomyopathy.

Bee L, Nasca A, Zanolini A, Cendron F, d'Adamo P, Costa R, Lamperti C, Celotti L, Ghezzi D, Zeviani M
EMBO molecular medicine 2015 Jul;7(7):918-29

Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.

Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature communications 2015 Aug 11;6:8015

DNA end resection is needed for the repair of complex lesions in G1-phase human cells.

Averbeck NB, Ringel O, Herrlitz M, Jakob B, Durante M, Taucher-Scholz G
Cell cycle (Georgetown, Tex.) 2014;13(16):2509-16

Rab5a is required for spindle length control and kinetochore-microtubule attachment during meiosis in oocytes.

Ma R, Hou X, Zhang L, Sun SC, Schedl T, Moley K, Wang Q
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Sep;28(9):4026-35

TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function.

Gómez-Herreros F, Schuurs-Hoeijmakers JH, McCormack M, Greally MT, Rulten S, Romero-Granados R, Counihan TJ, Chaila E, Conroy J, Ennis S, Delanty N, Cortés-Ledesma F, de Brouwer AP, Cavalleri GL, El-Khamisy SF, de Vries BB, Caldecott KW
Nature genetics 2014 May;46(5):516-21

Differential sensitivity of Glioma stem cells to Aurora kinase A inhibitors: implications for stem cell mitosis and centrosome dynamics.

Mannino M, Gomez-Roman N, Hochegger H, Chalmers AJ
Stem cell research 2014 Jul;13(1):135-43

DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities.

Shibata A, Moiani D, Arvai AS, Perry J, Harding SM, Genois MM, Maity R, van Rossum-Fikkert S, Kertokalio A, Romoli F, Ismail A, Ismalaj E, Petricci E, Neale MJ, Bristow RG, Masson JY, Wyman C, Jeggo PA, Tainer JA
Molecular cell 2014 Jan 9;53(1):7-18

The efficiency of homologous recombination and non-homologous end joining systems in repairing double-strand breaks during cell cycle progression.

Bee L, Fabris S, Cherubini R, Mognato M, Celotti L
PloS one 2013;8(7):e69061

Indicators of replicative damage in equine tendon fibroblast monolayers.

Rich T, Henderson LB, Becker DL, Cornell H, Patterson-Kane JC
BMC veterinary research 2013 Sep 11;9:180

The complexity of DNA double strand breaks is a critical factor enhancing end-resection.

Yajima H, Fujisawa H, Nakajima NI, Hirakawa H, Jeggo PA, Okayasu R, Fujimori A
DNA repair 2013 Nov;12(11):936-46

APLF promotes the assembly and activity of non-homologous end joining protein complexes.

Grundy GJ, Rulten SL, Zeng Z, Arribas-Bosacoma R, Iles N, Manley K, Oliver A, Caldecott KW
The EMBO journal 2013 Jan 9;32(1):112-25

Epstein-Barr virus-encoded latent membrane protein 1 impairs G2 checkpoint in human nasopharyngeal epithelial cells through defective Chk1 activation.

Deng W, Pang PS, Tsang CM, Hau PM, Yip YL, Cheung AL, Tsao SW
PloS one 2012;7(6):e39095

Nuclear envelope-associated dynein drives prophase centrosome separation and enables Eg5-independent bipolar spindle formation.

Raaijmakers JA, van Heesbeen RG, Meaders JL, Geers EF, Fernandez-Garcia B, Medema RH, Tanenbaum ME
The EMBO journal 2012 Nov 5;31(21):4179-90

Live-cell imaging visualizes frequent mitotic skipping during senescence-like growth arrest in mammary carcinoma cells exposed to ionizing radiation.

Suzuki M, Yamauchi M, Oka Y, Suzuki K, Yamashita S
International journal of radiation oncology, biology, physics 2012 Jun 1;83(2):e241-50

Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo.

Bui M, Dimitriadis EK, Hoischen C, An E, Quénet D, Giebe S, Nita-Lazar A, Diekmann S, Dalal Y
Cell 2012 Jul 20;150(2):317-26

Importin-β negatively regulates multiple aspects of mitosis including RANGAP1 recruitment to kinetochores.

Roscioli E, Di Francesco L, Bolognesi A, Giubettini M, Orlando S, Harel A, Schininà ME, Lavia P
The Journal of cell biology 2012 Feb 20;196(4):435-50

RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion.

Matsunaga S, Takata H, Morimoto A, Hayashihara K, Higashi T, Akatsuchi K, Mizusawa E, Yamakawa M, Ashida M, Matsunaga TM, Azuma T, Uchiyama S, Fukui K
Cell reports 2012 Apr 19;1(4):299-308

Discordance between phosphorylation and recruitment of 53BP1 in response to DNA double-strand breaks.

Harding SM, Bristow RG
Cell cycle (Georgetown, Tex.) 2012 Apr 1;11(7):1432-44

Survival of tumor cells after proton irradiation with ultra-high dose rates.

Auer S, Hable V, Greubel C, Drexler GA, Schmid TE, Belka C, Dollinger G, Friedl AA
Radiation oncology (London, England) 2011 Oct 18;6:139

Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA double-strand breaks in human cells.

Bauerschmidt C, Woodcock M, Stevens DL, Hill MA, Rothkamm K, Helleday T
Experimental cell research 2011 Feb 1;317(3):330-7

ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome Congression.

Lee MH, Lin L, Equilibrina I, Uchiyama S, Matsunaga S, Fukui K
Acta histochemica et cytochemica 2011 Dec 28;44(6):247-58

Cohesin promotes the repair of ionizing radiation-induced DNA double-strand breaks in replicated chromatin.

Bauerschmidt C, Arrichiello C, Burdak-Rothkamm S, Woodcock M, Hill MA, Stevens DL, Rothkamm K
Nucleic acids research 2010 Jan;38(2):477-87

Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma.

Ladstein RG, Bachmann IM, Straume O, Akslen LA
BMC cancer 2010 Apr 14;10:140

Probing PML body function in ALT cells reveals spatiotemporal requirements for telomere recombination.

Draskovic I, Arnoult N, Steiner V, Bacchetti S, Lomonte P, Londoño-Vallejo A
Proceedings of the National Academy of Sciences of the United States of America 2009 Sep 15;106(37):15726-31

Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage.

Toralová T, Susor A, Nemcová L, Kepková K, Kanka J
Reproduction (Cambridge, England) 2009 Nov;138(5):783-91

The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells.

Helbling Chadwick L, Chadwick BP, Jaye DL, Wade PA
Chromosoma 2009 Aug;118(4):445-57

PinX1 is a novel microtubule-binding protein essential for accurate chromosome segregation.

Yuan K, Li N, Jiang K, Zhu T, Huo Y, Wang C, Lu J, Shaw A, Thomas K, Zhang J, Mann D, Liao J, Jin C, Yao X
The Journal of biological chemistry 2009 Aug 21;284(34):23072-82

Activation of FoxM1 during G2 requires cyclin A/Cdk-dependent relief of autorepression by the FoxM1 N-terminal domain.

Laoukili J, Alvarez M, Meijer LA, Stahl M, Mohammed S, Kleij L, Heck AJ, Medema RH
Molecular and cellular biology 2008 May;28(9):3076-87

Evidence for the receipt of DNA damage stimuli by PML nuclear domains.

Varadaraj A, Dovey CL, Laredj L, Ferguson B, Alexander CE, Lubben N, Wyllie AH, Rich T
The Journal of pathology 2007 Mar;211(4):471-80

Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase.

Bauerschmidt C, Pollok S, Kremmer E, Nasheuer HP, Grosse F
Genes to cells : devoted to molecular & cellular mechanisms 2007 Jun;12(6):745-58

Human Cdc45 is a proliferation-associated antigen.

Pollok S, Bauerschmidt C, Sänger J, Nasheuer HP, Grosse F
The FEBS journal 2007 Jul;274(14):3669-3684

CENP-F expression is associated with poor prognosis and chromosomal instability in patients with primary breast cancer.

O'Brien SL, Fagan A, Fox EJ, Millikan RC, Culhane AC, Brennan DJ, McCann AH, Hegarty S, Moyna S, Duffy MJ, Higgins DG, Jirström K, Landberg G, Gallagher WM
International journal of cancer 2007 Apr 1;120(7):1434-43

Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins.

Maeshima K, Yahata K, Sasaki Y, Nakatomi R, Tachibana T, Hashikawa T, Imamoto F, Imamoto N
Journal of cell science 2006 Nov 1;119(Pt 21):4442-51

Sgt1 is required for human kinetochore assembly.

Steensgaard P, Garrè M, Muradore I, Transidico P, Nigg EA, Kitagawa K, Earnshaw WC, Faretta M, Musacchio A
EMBO reports 2004 Jun;5(6):626-31

Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis.

Sorensen CS, Lukas C, Kramer ER, Peters JM, Bartek J, Lukas J
Molecular and cellular biology 2000 Oct;20(20):7613-23

Nonperiodic activity of the human anaphase-promoting complex-Cdh1 ubiquitin ligase results in continuous DNA synthesis uncoupled from mitosis.

Sorensen CS, Lukas C, Kramer ER, Peters JM, Bartek J, Lukas J
Molecular and cellular biology 2000 Oct;20(20):7613-23

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.