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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX105029 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105029, RRID:AB_11179121
- Product name
- TFIIE beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat nucleus 10% SDS PAGE GTX105029 diluted at 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 £gg whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 £gg of TFIIE beta antibody (GTX105029) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (GTX105029) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 £gg of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
