MA1-845

antibody from Invitrogen Antibodies

Targeting: NCOA3 ACTR, AIB1, bHLHe42, CAGH16, KAT13B, p/CIP, RAC3, SRC-3, SRC3, TNRC16, TRAM-1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-845

Provider

Invitrogen Antibodies

Product name

SRC3 Monoclonal Antibody (AX15.3)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-845 detects amplified in breast cancer-1 (AIB1) in human cells. MA1-845 has been successfully used in Western blot and immunoprecipitation, ChIP, and immunohistochemistry procedures. By Western blot, this antibody detects an ~155 kDa protein representing AIB1 from MCF-7 cell extract. The MA1-845 immunizing peptide corresponds to amino acid residues 605-1294 of human AIB1.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AX15.3

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Notch-1 activates estrogen receptor-alpha-dependent transcription via IKKalpha in breast cancer cells.

Hao L, Rizzo P, Osipo C, Pannuti A, Wyatt D, Cheung LW, Sonenshein G, Osborne BA, Miele L
Oncogene 2010 Jan 14;29(2):201-13

---

Progressive loss of estrogen receptor alpha cofactor recruitment in endocrine resistance.

Naughton C, MacLeod K, Kuske B, Clarke R, Cameron DA, Langdon SP
Molecular endocrinology (Baltimore, Md.) 2007 Nov;21(11):2615-26

---

Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation.

Park KJ, Krishnan V, O'Malley BW, Yamamoto Y, Gaynor RB
Molecular cell 2005 Apr 1;18(1):71-82

---

Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in vitamin D receptor transactivation during keratinocyte differentiation.

Oda Y, Sihlbom C, Chalkley RJ, Huang L, Rachez C, Chang CP, Burlingame AL, Freedman LP, Bikle DD
Molecular endocrinology (Baltimore, Md.) 2003 Nov;17(11):2329-39

---

Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1.

Azorsa DO, Meltzer PS
Hybridoma 1999 Jun;18(3):281-7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of AIB1 on MCF-7 cell extract using Product # MA1-845.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a SRC3 monoclonal antibody (Product # MA1-845) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting