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LearnNBP1-77785
antibody from Novus Biologicals
Targeting: NCOA3
ACTR, AIB1, bHLHe42, CAGH16, KAT13B, p/CIP, RAC3, SRC-3, SRC3, TNRC16, TRAM-1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-77785 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77785, RRID:AB_11005740
- Product name
- Rabbit Polyclonal NCOA3/AIB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with several isoforms of human AIB1 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with AIB1 from h
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.24 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to AIB1. Mouse AIB1(lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
