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LearnNB100-315
antibody from Novus Biologicals
Targeting: NCOA3
ACTR, AIB1, bHLHe42, CAGH16, KAT13B, p/CIP, RAC3, SRC-3, SRC3, TNRC16, TRAM-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-315 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-315, RRID:AB_2151065
- Product name
- Rabbit Polyclonal NCOA3/AIB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Samples: Nuclear extract (5 ug for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 ug/ml for WB and 20 ug/10 mg extract for CoIP. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min. for WB and Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3 for CoIP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 (SRC3) by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCOA3/AIB1 Antibody [NB100-315] - Detection of human NCOA3/AIB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCOA3/AIB1 antibody NB100-315 (lot 2) used for IP at 6 ug per reaction. NCOA3/AIB1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated NCOA3/AIB1, NB100-315 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
