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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PP1320 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#PP1320, RRID:AB_1002193
- Product name
- anti Heparanase / HPSE
- Antibody type
- Polyclonal
- Antigen
- 50 kDa-8 kDa Heparanase heterodimer
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.24 mg
- Concentration
- 2.4 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- An extract from 6.5 x 10e3 CHO cells, transfected with human Heparanase, extract from 8 x 10e4 CHO cells, transfected with mouse Heparanase, and an extract from 5 x 10e5 non-transfected CHO dhfr- cells, were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF membrane and subjected to Western blot analysis using 1:1000 polyclonal rabbit anti-human HPA1. Purified recombinant human heparanase served as a control (left lane), where the 65-kDa precursor and the 50-kDa and 8-kDa subunits are clearly detected.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HUMAN PLACENTA x 400. Paraffin sections of human placenta were stained with 1:100 of polyclonal rabbit anti-human HPA1 (B) or without Poly Ab (A).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- BLOOD SMEAR x1000. Human blood smears were stained with 1:100 of polyclonal rabbit anti-human HPA1(B) or without Poly Ab (A). Note the strong staining of Neutrophils (N), while lymphocytes (L) are not stained.
