GTX114209

antibody from GeneTex

Targeting: SFPQ PPP1R140, PSF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX114209

Provider

GeneTex

Proper citation

GeneTex Cat#GTX114209, RRID:AB_11179808

Product name

SFPQ antibody [N2C1], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX114209 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE GTX114209 diluted at 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody detects SFPQ protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 £gg of SFPQ antibody [N2C1], Internal (GTX114209).Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SFPQ(GTX114209) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse uterus, using SFPQ(GTX114209) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.