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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- GTX70294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70294, RRID:AB_373299
- Product name
- XPC antibody [3.26]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references Xeroderma Pigmentosum Group C Deficiency Alters Cigarette Smoke DNA Damage Cell Fate and Accelerates Emphysema Development.
Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
Deficient Nucleotide Excision Repair in Squamous Cell Carcinoma Cells.
Transcriptional repressor ZBTB1 promotes chromatin remodeling and translesion DNA synthesis.
The RecQ helicase RECQL5 participates in psoralen-induced interstrand cross-link repair.
Defective DNA repair and cell cycle arrest in cells expressing Merkel cell polyomavirus T antigen.
DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.
Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes.
The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas.
Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells.
SUMO-dependent regulation of centrin-2.
Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells.
Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair.
Impaired repair of cyclobutane pyrimidine dimers in human keratinocytes deficient in p53 and p63.
Increased apoptosis, p53 up-regulation, and cerebellar neuronal degeneration in repair-deficient Cockayne syndrome mice.
Proficient global nucleotide excision repair in human keratinocytes but not in fibroblasts deficient in p53.
p53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene.
Sears CR, Zhou H, Justice MJ, Fisher AJ, Saliba J, Lamb I, Wicker J, Schweitzer KS, Petrache I
American journal of respiratory cell and molecular biology 2018 Mar;58(3):402-411
American journal of respiratory cell and molecular biology 2018 Mar;58(3):402-411
Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
Jia L, Yan F, Cao W, Chen Z, Zheng H, Li H, Pan Y, Narula N, Ren X, Li H, Zhou P
The Journal of biological chemistry 2017 Feb 17;292(7):2966-2978
The Journal of biological chemistry 2017 Feb 17;292(7):2966-2978
Deficient Nucleotide Excision Repair in Squamous Cell Carcinoma Cells.
Dong TK, Ona K, Scandurra AE, Demetriou SK, Oh DH
Photochemistry and photobiology 2016 Sep;92(5):760-6
Photochemistry and photobiology 2016 Sep;92(5):760-6
Transcriptional repressor ZBTB1 promotes chromatin remodeling and translesion DNA synthesis.
Kim H, Dejsuphong D, Adelmant G, Ceccaldi R, Yang K, Marto JA, D'Andrea AD
Molecular cell 2014 Apr 10;54(1):107-118
Molecular cell 2014 Apr 10;54(1):107-118
The RecQ helicase RECQL5 participates in psoralen-induced interstrand cross-link repair.
Ramamoorthy M, May A, Tadokoro T, Popuri V, Seidman MM, Croteau DL, Bohr VA
Carcinogenesis 2013 Oct;34(10):2218-30
Carcinogenesis 2013 Oct;34(10):2218-30
Defective DNA repair and cell cycle arrest in cells expressing Merkel cell polyomavirus T antigen.
Demetriou SK, Ona-Vu K, Sullivan EM, Dong TK, Hsu SW, Oh DH
International journal of cancer 2012 Oct 15;131(8):1818-27
International journal of cancer 2012 Oct 15;131(8):1818-27
DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.
Takedachi A, Saijo M, Tanaka K
Molecular and cellular biology 2010 Jun;30(11):2708-23
Molecular and cellular biology 2010 Jun;30(11):2708-23
Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes.
Rezvani HR, Mahfouf W, Ali N, Chemin C, Ged C, Kim AL, de Verneuil H, Taïeb A, Bickers DR, Mazurier F
Nucleic acids research 2010 Jan;38(3):797-809
Nucleic acids research 2010 Jan;38(3):797-809
The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas.
de Feraudy S, Ridd K, Richards LM, Kwok PY, Revet I, Oh D, Feeney L, Cleaver JE
The American journal of pathology 2010 Aug;177(2):555-62
The American journal of pathology 2010 Aug;177(2):555-62
Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells.
Acu ID, Liu T, Suino-Powell K, Mooney SM, D'Assoro AB, Rowland N, Muotri AR, Correa RG, Niu Y, Kumar R, Salisbury JL
Cancer research 2010 Apr 15;70(8):3320-8
Cancer research 2010 Apr 15;70(8):3320-8
SUMO-dependent regulation of centrin-2.
Klein UR, Nigg EA
Journal of cell science 2009 Sep 15;122(Pt 18):3312-21
Journal of cell science 2009 Sep 15;122(Pt 18):3312-21
Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells.
Yan T, Seo Y, Kinsella TJ
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6912-20
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Nov 15;15(22):6912-20
Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair.
Wang Y, Liu X, Matsuda A, Plunkett W
Cancer research 2008 May 15;68(10):3881-9
Cancer research 2008 May 15;68(10):3881-9
Impaired repair of cyclobutane pyrimidine dimers in human keratinocytes deficient in p53 and p63.
Ferguson-Yates BE, Li H, Dong TK, Hsiao JL, Oh DH
Carcinogenesis 2008 Jan;29(1):70-5
Carcinogenesis 2008 Jan;29(1):70-5
Increased apoptosis, p53 up-regulation, and cerebellar neuronal degeneration in repair-deficient Cockayne syndrome mice.
Laposa RR, Huang EJ, Cleaver JE
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 23;104(4):1389-94
Proceedings of the National Academy of Sciences of the United States of America 2007 Jan 23;104(4):1389-94
Proficient global nucleotide excision repair in human keratinocytes but not in fibroblasts deficient in p53.
Ferguson BE, Oh DH
Cancer research 2005 Oct 1;65(19):8723-9
Cancer research 2005 Oct 1;65(19):8723-9
p53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene.
Adimoolam S, Ford JM
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12985-90
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12985-90
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot image of human XPC from HeLa whole cell lysate detected using rabbit polyclonal (lanes 1 and 2, catalog numbers GTX70308 and GTX70309, respectively) or monoclonal (lane 3, catalog number GTX70294) antibodies.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of human XPC protein using anti-XPC 3.26 monoclonal antibody (GTX70294) in Raji whole cell extract. Lane 1: 1:1,000 dilution. Lane 2: 1:500 dilution.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XPC antibody [3.26] detects XPC protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] (GTX70294) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XPC antibody [3.26] detects XPC protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: XPC protein stained by XPC antibody [3.26] (GTX70294) diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
