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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX110646 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110646, RRID:AB_1951582
- Product name
- RAB6A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Upregulation of microRNA-4417 and Its Target Genes Contribute to Nickel Chloride-promoted Lung Epithelial Cell Fibrogenesis and Tumorigenesis.
Rab6 regulation of the kinesin family KIF1C motor domain contributes to Golgi tethering.
Wu CH, Hsiao YM, Yeh KT, Tsou TC, Chen CY, Wu MF, Ko JL
Scientific reports 2017 Nov 10;7(1):15320
Scientific reports 2017 Nov 10;7(1):15320
Rab6 regulation of the kinesin family KIF1C motor domain contributes to Golgi tethering.
Lee PL, Ohlson MB, Pfeffer SR
eLife 2015 Mar 30;4
eLife 2015 Mar 30;4
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE GTX110646 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein by western blot analysis.A. 50 £gg mouse brain lysate/extract12 % SDS-PAGERAB6A antibody (GTX110646) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein by western blot analysis.A. 50 £gg rat brain cell lysate/extract12 % SDS-PAGERAB6A antibody (GTX110646) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein by Western blot analysis.A. 50 £gg mouse brain lysate/extract12 % SDS-PAGERAB6A antibody (GTX110646) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein by Western blot analysis.A. 50 £gg rat brain lysate/extract12 % SDS-PAGERAB6A antibody (GTX110646) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein by Western blot analysis.A. 30 £gg A549 whole cell lysate/extract B. 30 £gg H1299 whole cell lysate/extract C. 30 £gg HCT116 whole cell lysate/extract12 % SDS-PAGERAB6A antibody (GTX110646) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody (GTX110646) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using RAB6A(GTX110646) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAB6A antibody detects RAB6A protein at Golgi apparatus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB6A protein stained by RAB6A antibody (GTX110646) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using RAB6A(GTX110646) antibody at 1:500 dilution.
