10187-2-AP

antibody from Invitrogen Antibodies

Targeting: RAB6A RAB6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10187-2-AP

Provider

Invitrogen Antibodies

Product name

RAB6A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSTGGDFGN PLRKFKLVFL GEQSVGKTSL ITRFMYDSFD NTYQATIGID FLSKTMYLED RTIRLQLWDT AGQERFRSLI PSYIRDSAAA VVVYDITNVN SFQQTTKWID DVRTERGSDV IIMLVGNKTD LADKRQVSIE EGERKAKELN VMFIETSAKA GYNVKQLFRR VAAALPGMES TQDRSREDMI DIKLEKPQEQ PVSEGGCSC (1-208 aa encoded by BC003617 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10187-2-AP (RAB6A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 10187-2-AP (RAB6A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10187-2-AP (RAB6A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 10187-2-AP) RAB6A antibody, at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10187-2-AP (RAB6A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RAB6A (IP:10187-2-AP, 4ug; Detection:10187-2-AP 1:500) with mouse brain tissue lysate 2640ug.