MA5-34736

antibody from Invitrogen Antibodies

Targeting: PRPF8 hPrp8, Prp8, PRPC8, RP13, SNRNP220

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34736

Provider

Invitrogen Antibodies

Product name

PRP8 Recombinant Rabbit Monoclonal Antibody (JG57-38)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, 293T, LOVO, SiHa, rat brain tissue, human colon tissue, human prostate cancer tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG57-38

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRPF8 in SiHa (1) and A549 (2) cell lysate. Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRPF8 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRPF8 monoclonal antibody (Product # MA5-34736), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRPF8 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRPF8 monoclonal antibody (Product # MA5-34736), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PRPF8 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with PRPF8 monoclonal antibody (Product # MA5-34736), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRPF8 in paraffin-embedded rat brain tissue. Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRPF8 in paraffin-embedded mouse testis tissue. Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRPF8 in paraffin-embedded human prostate cancer tissue. Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRPF8 in paraffin-embedded human colon tissue. Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PRPF8 in A549 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with PRPF8 monoclonal antibody (Product # MA5-34736) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.