AF1539

antibody from R&D Systems

Targeting: MSTN GDF8

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1539

Provider

R&D Systems

Product name

Mouse GDF-8/Myostatin Propeptide Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects GDF-8/Myostatin Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with mature recombinant mouse (rm) GDF-8 is observed and less than 5% cross-reactivity with mature rmGDF-1, -3, -5, -6, -7, -9, and recombinant human GDF-11 is observed.

Reactivity

Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O08689


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Blocking extracellular activation of myostatin as a strategy for treating muscle wasting.

Pirruccello-Straub M, Jackson J, Wawersik S, Webster MT, Salta L, Long K, McConaughy W, Capili A, Boston C, Carven GJ, Mahanthappa NK, Turner KJ, Donovan A
Scientific reports 2018 Feb 2;8(1):2292

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The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration.

Miroshnychenko O, Chang WT, Dragoo JL
The American journal of sports medicine 2017 Mar;45(4):945-953

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Myostatin blockade with a fully human monoclonal antibody induces muscle hypertrophy and reverses muscle atrophy in young and aged mice.

Latres E, Pangilinan J, Miloscio L, Bauerlein R, Na E, Potocky TB, Huang Y, Eckersdorff M, Rafique A, Mastaitis J, Lin C, Murphy AJ, Yancopoulos GD, Gromada J, Stitt T
Skeletal muscle 2015;5:34

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A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle.

Dubois V, Laurent MR, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, Spans L, Gayan-Ramirez G, Deldicque L, Hespel P, Carmeliet G, Vanderschueren D, Claessens F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Jul;28(7):2979-94

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Myostatin expression, lymphocyte population, and potential cytokine production correlate with predisposition to high-fat diet induced obesity in mice.

Lyons JA, Haring JS, Biga PR
PloS one 2010 Sep 22;5(9):e12928

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Targeting of bone morphogenetic protein growth factor complexes to fibrillin.

Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bächinger HP, Sakai LY
The Journal of biological chemistry 2008 May 16;283(20):13874-88

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Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition.

Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y
The Journal of clinical investigation 2006 Nov;116(11):2924-34

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GDF-8 Propeptide Inhibition of GDF-8/Myostatin-induced Hemoglobin Expression and Neutralization by Mouse GDF-8 Propeptide Antibody. Recombinant Mouse GDF-8 Propeptide (Catalog # 1539-PG) inhibits Recombinant Mouse GDF-8/Myostatin (Catalog # 788-G8) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Inhibition of Recombinant Mouse GDF-8/Myostatin (40 ng/mL) activity elicited by Recombinant Mouse GDF-8 Propeptide (800 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse GDF-8/Myostatin Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1539). The ND₅₀ is typically 10-30 µg/mL.