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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF2570 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nidogen-1/Entactin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Nidogen-1/Entactin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAH45606- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Descemet's Membrane Modulation of Posterior Corneal Fibrosis.
IL-1 and TGF-β Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells.
Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition.
Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
Medeiros CS, Saikia P, de Oliveira RC, Lassance L, Santhiago MR, Wilson SE
Investigative ophthalmology & visual science 2019 Mar 1;60(4):1010-1020
Investigative ophthalmology & visual science 2019 Mar 1;60(4):1010-1020
IL-1 and TGF-β Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells.
Saikia P, Thangavadivel S, Medeiros CS, Lassance L, de Oliveira RC, Wilson SE
Investigative ophthalmology & visual science 2018 Nov 1;59(13):5589-5598
Investigative ophthalmology & visual science 2018 Nov 1;59(13):5589-5598
Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity.
Mattsson JM, Ravela S, Hekim C, Jonsson M, Malm J, Närvänen A, Stenman UH, Koistinen H
PloS one 2014;9(9):e107819
PloS one 2014;9(9):e107819
Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions.
Smith AO, Bowers SL, Stratman AN, Davis GE
PloS one 2013;8(12):e85147
PloS one 2013;8(12):e85147
Development and characterization of a high-throughput in vitro cord formation model insensitive to VEGF inhibition.
Falcon BL, O'Clair B, McClure D, Evans GF, Stewart J, Swearingen ML, Chen Y, Allard K, Lee LN, Neote K, McEwen DP, Uhlik MT, Chintharlapalli S
Journal of hematology & oncology 2013 Apr 27;6:31
Journal of hematology & oncology 2013 Apr 27;6:31
Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation.
Stratman AN, Malotte KM, Mahan RD, Davis MJ, Davis GE
Blood 2009 Dec 3;114(24):5091-101
Blood 2009 Dec 3;114(24):5091-101
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nidogen-1/Entactin in Human Heart. Nidogen-1/Entactin was detected in immersion fixed paraffin-embedded sections of human heart using Goat Anti-Human Nidogen-1/Entactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2570) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcolemma of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
