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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF8006 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Endophilin A1/SH3GL2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Endophilin A1/SH3GL2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q99962- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat Endophilin A1/SH3GL2 by Western Blot. Western blot shows lysates of human brain stem tissue, human brain (hypothalamus) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Endophilin A1/SH3GL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8006) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Endophilin A1/SH3GL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
