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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB0250 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0250, RRID:AB_1019044
- Product name
- CASP14 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CASP14.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phospho-ΔNp63α regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation.
Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Ratovitski EA
FEBS letters 2013 Nov 1;587(21):3581-6
FEBS letters 2013 Nov 1;587(21):3581-6
Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S
Experimental neurology 2004 Oct;189(2):261-79
Experimental neurology 2004 Oct;189(2):261-79
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP14. Tissue lysates (50 ug/lane) and recombinant human CASP14 were (Hu C14, 15 ng) were western blotted with CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. The antisera detected both the proform of CASP14, and the large and small subunits of active/cleaved CASP14.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1) . Decreased CASP14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded tissue sections of human cervix stained for CASP14 expression. Using CASP14 polyclonal antibody (Cat # PAB0250) at 1 : 2000. A. Normal cervix (squamous epithelium) . B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia) . C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia) . D. CIN3 (high-grade squamous intraepithelial lesion ; severe dysplasia-carcinoma in situ) . In normal cervi, CASP14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of CASP14 expression with differentiation. CASP14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
