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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP21654SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21654SU-S, RRID:AB_10756375
- Product name
- anti Caspase-14
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length human Caspase-14 was used as immunogen.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-14. Tissue lysates (50 µg/lane) and recombinant human caspase-14 were (Hu C14, 15 ng) were western blotted with anti-Caspase-14 (AP21654SU-S) at 1/2000. The antisera detected both the proform of caspase-14, and the large and small subunits of active/cleaved caspase-14.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded tissue sections of human cervix stained for Caspase-14 expression using AP21654SU-S at 1/2000. A. Normal cervix (squamous epithelium). B. CIN1 (low-grade squamous intraepithelial lesion, mild dysplasia). C. CIN2 (high-grade squamous intraepithelial lesion, moderate dysplasia. D. CIN3 (high-grade squamous intraepithelial lesion; severe dysplasia-carcinoma in situ. In normal cervi, caspase-14 staining was found most in the midzone layer, but was absent from the basal/parabasal cell layer where mitotically active cells are known to reside. This suggests induction of caspase-14 expression with differentiation. Caspase-14 expression declined progressively during malignant transformation as the histologic severity of the cervical atypia advanced from CIN1 to CIN3. Hematoxylin-eosin counterstain.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for Caspase-14 expression using AP21654SU-S at 1/2000. Low (A) and high (B) stage ovarian tumor tissue cores. High magnification from areas of the tissue cores (A1 and B1). Decreased Caspase-14 expression was seen in the high grade, compared to the low grade tumor. Hematoxylin-eosin counterstain.
