619304

antibody from BioLegend

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

619304

Provider

BioLegend

Proper citation

BioLegend Cat#619304, RRID:AB_2563298

Product name

Alexa Fluor® 594 anti-TIF1β (KAP-1, TRIM28)

Antibody type

Monoclonal

Antigen

Other

Description

TIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits setdbl to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with setdbl and cbx3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

20A1

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-TIF1β (clone 20A1) Alexa Fluor® 594 (red) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.

Conjugate

Yellow dye