M00409-2

antibody from Boster Biological Technology

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00409-2

Provider

Boster Biological Technology

Product name

Anti-KAP1/TRIM28 Antibody Picoband™ (monoclonal, 9E3)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for KAP1/TRIM28 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

9.00E+03

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of KAP1/TRIM28 using anti-KAP1/TRIM28 antibody (M00409-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human PC-3 whole cell lysates; Lane 3: human HEK293 whole cell lysates; Lane 4: human A549 whole cell lysates; Lane 5: human Jurkat whole cell lysates; Lane 6: human THP-1 whole cell lysates; Lane 7: rat PC-12 whole cell lysates; Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KAP1/TRIM28 antigen affinity purified monoclonal antibody (Catalog # M00409-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KAP1/TRIM28 at approximately 100KD. The expected band size for KAP1/TRIM28 is at 100KD.

Additional image