NB100-2350

antibody from Novus Biologicals

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2350

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2350, RRID:AB_10002723

Product name

Rabbit Polyclonal KAP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Rats with a missense mutation in Atm display neuroinflammation and neurodegeneration subsequent to accumulation of cytosolic DNA following unrepaired DNA damage.

Quek H, Luff J, Cheung K, Kozlov S, Gatei M, Lee CS, Bellingham MC, Noakes PG, Lim YC, Barnett NL, Dingwall S, Wolvetang E, Mashimo T, Roberts TL, Lavin MF
Journal of leukocyte biology 2017 Apr;101(4):927-947

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Recruitment and activation of the ATM kinase in the absence of DNA-damage sensors.

Hartlerode AJ, Morgan MJ, Wu Y, Buis J, Ferguson DO
Nature structural & molecular biology 2015 Sep;22(9):736-43

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ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1.

Kijas AW, Lim YC, Bolderson E, Cerosaletti K, Gatei M, Jakob B, Tobias F, Taucher-Scholz G, Gueven N, Oakley G, Concannon P, Wolvetang E, Khanna KK, Wiesmüller L, Lavin MF
Nucleic acids research 2015 Sep 30;43(17):8352-67

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MAGE-C2 promotes growth and tumorigenicity of melanoma cells, phosphorylation of KAP1, and DNA damage repair.

Bhatia N, Xiao TZ, Rosenthal KA, Siddiqui IA, Thiyagarajan S, Smart B, Meng Q, Zuleger CL, Mukhtar H, Kenney SC, Albertini MR, Jack Longley B
The Journal of investigative dermatology 2013 Mar;133(3):759-767

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Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice.

Puccini J, Shalini S, Voss AK, Gatei M, Wilson CH, Hiwase DK, Lavin MF, Dorstyn L, Kumar S
Proceedings of the National Academy of Sciences of the United States of America 2013 Dec 3;110(49):19920-5

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Phosphoproteomic analysis reveals that PP4 dephosphorylates KAP-1 impacting the DNA damage response.

Lee DH, Goodarzi AA, Adelmant GO, Pan Y, Jeggo PA, Marto JA, Chowdhury D
The EMBO journal 2012 May 16;31(10):2403-15

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Temporally distinct translesion synthesis pathways for ultraviolet light-induced photoproducts in the mammalian genome.

Temviriyanukul P, van Hees-Stuivenberg S, Delbos F, Jacobs H, de Wind N, Jansen JG
DNA repair 2012 Jun 1;11(6):550-8

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Targeting radiation-resistant hypoxic tumour cells through ATR inhibition.

Pires IM, Olcina MM, Anbalagan S, Pollard JR, Reaper PM, Charlton PA, McKenna WG, Hammond EM
British journal of cancer 2012 Jul 10;107(2):291-9

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Kinase-dead ATM protein causes genomic instability and early embryonic lethality in mice.

Yamamoto K, Wang Y, Jiang W, Liu X, Dubois RL, Lin CS, Ludwig T, Bakkenist CJ, Zha S
The Journal of cell biology 2012 Aug 6;198(3):305-13

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Kruppel-associated Box (KRAB)-associated co-repressor (KAP-1) Ser-473 phosphorylation regulates heterochromatin protein 1β (HP1-β) mobilization and DNA repair in heterochromatin.

Bolderson E, Savage KI, Mahen R, Pisupati V, Graham ME, Richard DJ, Robinson PJ, Venkitaraman AR, Khanna KK
The Journal of biological chemistry 2012 Aug 10;287(33):28122-31

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MAGE I transcription factors regulate KAP1 and KRAB domain zinc finger transcription factor mediated gene repression.

Xiao TZ, Bhatia N, Urrutia R, Lomberk GA, Simpson A, Longley BJ
PloS one 2011;6(8):e23747

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Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin.

Sims JK, Wade PA
Molecular biology of the cell 2011 Sep;22(17):3094-102

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ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control.

Gatei M, Jakob B, Chen P, Kijas AW, Becherel OJ, Gueven N, Birrell G, Lee JH, Paull TT, Lerenthal Y, Fazry S, Taucher-Scholz G, Kalb R, Schindler D, Waltes R, Dörk T, Lavin MF
The Journal of biological chemistry 2011 Sep 9;286(36):31542-56

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Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection.

Forrester NA, Sedgwick GG, Thomas A, Blackford AN, Speiseder T, Dobner T, Byrd PJ, Stewart GS, Turnell AS, Grand RJ
Journal of virology 2011 Mar;85(5):2201-11

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A hypomorphic Artemis human disease allele causes aberrant chromosomal rearrangements and tumorigenesis.

Jacobs C, Huang Y, Masud T, Lu W, Westfield G, Giblin W, Sekiguchi JM
Human molecular genetics 2011 Feb 15;20(4):806-19

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Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum.

Du L, Kayali R, Bertoni C, Fike F, Hu H, Iversen PL, Gatti RA
Human molecular genetics 2011 Aug 15;20(16):3151-60

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Identification of novel small molecules that inhibit protein-protein interactions between MAGE and KAP-1.

Bhatia N, Yang B, Xiao TZ, Peters N, Hoffmann MF, Longley BJ
Archives of biochemistry and biophysics 2011 Apr 15;508(2):217-21

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Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways.

Gilmore-Hebert M, Ramabhadran R, Stern DF
Molecular cancer research : MCR 2010 Oct;8(10):1388-98

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The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain.

Kanu N, Penicud K, Hristova M, Wong B, Irvine E, Plattner F, Raivich G, Behrens A
The Journal of biological chemistry 2010 Dec 3;285(49):38534-42

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The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks.

Bansbach CE, Bétous R, Lovejoy CA, Glick GG, Cortez D
Genes & development 2009 Oct 15;23(20):2405-14

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Functional genomic screens identify CINP as a genome maintenance protein.

Lovejoy CA, Xu X, Bansbach CE, Glick GG, Zhao R, Ye F, Sirbu BM, Titus LC, Shyr Y, Cortez D
Proceedings of the National Academy of Sciences of the United States of America 2009 Nov 17;106(46):19304-9

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MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks.

Helmink BA, Bredemeyer AL, Lee BS, Huang CY, Sharma GG, Walker LM, Bednarski JJ, Lee WL, Pandita TK, Bassing CH, Sleckman BP
The Journal of experimental medicine 2009 Mar 16;206(3):669-79

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Distinct roles of ATR and DNA-PKcs in triggering DNA damage responses in ATM-deficient cells.

Tomimatsu N, Mukherjee B, Burma S
EMBO reports 2009 Jun;10(6):629-35

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Kruppel-associated box domain-associated protein-1 as a latency regulator for Kaposi's sarcoma-associated herpesvirus and its modulation by the viral protein kinase.

Chang PC, Fitzgerald LD, Van Geelen A, Izumiya Y, Ellison TJ, Wang DH, Ann DK, Luciw PA, Kung HJ
Cancer research 2009 Jul 15;69(14):5681-9

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ATM activation and signaling under hypoxic conditions.

Bencokova Z, Kaufmann MR, Pires IM, Lecane PS, Giaccia AJ, Hammond EM
Molecular and cellular biology 2009 Jan;29(2):526-37

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Autophosphorylation at serine 1981 stabilizes ATM at DNA damage sites.

So S, Davis AJ, Chen DJ
The Journal of cell biology 2009 Dec 28;187(7):977-90

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Multiple autophosphorylation sites are dispensable for murine ATM activation in vivo.

Daniel JA, Pellegrini M, Lee JH, Paull TT, Feigenbaum L, Nussenzweig A
The Journal of cell biology 2008 Dec 1;183(5):777-83

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ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells.

Biton S, Gropp M, Itsykson P, Pereg Y, Mittelman L, Johe K, Reubinoff B, Shiloh Y
DNA repair 2007 Jan 4;6(1):128-34

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Role for KAP1 serine 824 phosphorylation and sumoylation/desumoylation switch in regulating KAP1-mediated transcriptional repression.

Li X, Lee YK, Jeng JC, Yen Y, Schultz DC, Shih HM, Ann DK
The Journal of biological chemistry 2007 Dec 14;282(50):36177-89

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Detection Human Phospho KAP-1 (S824) on HeLa whole cell lysate. KAP-1 was immunoprecipitated using another rabbit anti-KAP-1 antibody BL553.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Whole cell lysate (15 and 50 ug) from 293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows Western Blot for total KAP1-1 using affinity purified rabbit antiKAP-1 antibody at 0.1 ug/ml with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NB100-2350 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second. Lower panel shows western blot for total KAP-1 using affinity purified rabbit anti KAP-1 antibody at 0.1 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal antibody at 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Detection of human Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) NB100-2350 and rabbit anti-KAP-1 recombinant monoclonal antibody NBP2-76393 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Phospho KAP-1 (S824), NB100-2350 was used at 0.04 ug/ml. To examine total KAP-1, the blot was stripped and then blotted with NBP2-76393 at 1:1000 (lower panel). Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) and untreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB