STJ72911

antibody from St John's Laboratory

Targeting: CSTF2 CstF-64

Immunoprecipitation

Antibody data

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Validation data

CSTF2 protein structure - STJ72911 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse ßCstf-64 (DYKDDDDK tagged) immunoprecipitated using STJ72911 (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ72911 (2ug) used to pull down ßCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-ßCstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA