MA5-34933

antibody from Invitrogen Antibodies

Targeting: STMN1 C1orf215, FLJ32206, Lag, LAP18, OP18, PP17, PP19, PR22, SMN

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34933

Provider

Invitrogen Antibodies

Product name

Stathmin 1 Monoclonal Antibody (11E1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: MG-63, A431, Human tonsil tissue, human colon cancer tissue, human breast cancer tissue.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

11E1

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Stathmin 1 in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:200 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Stathmin 1 in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:200 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Stathmin 1 in paraffin-embedded human tonsil tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Stathmin 1 in paraffin-embedded human colon tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Stathmin 1 in paraffin-embedded human breast cancer tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Stathmin 1 in MG-63 cells, (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Stathmin 1 monoclonal antibody (Product # MA5-34933) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG with a dilution of 1:500.