- PA5-50263 - Invitrogen Antibodies SOCS6 antibody

Submitted references MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death.

Fu B, Lin X, Tan S, Zhang R, Xue W, Zhang H, Zhang S, Zhao Q, Wang Y, Feldman K, Shi L, Zhang S, Nian W, Chaitanya Pavani K, Li Z, Wang X, Wu H
EMBO reports 2021 Sep 6;22(9):e52252

A short deletion in the DNA-binding domain of STAT3 suppresses growth and progression of colon cancer cells.

Xiong YJ, Liu DY, Shen RR, Xiong Y
Aging 2021 Feb 1;13(4):5185-5196

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of SOCS6 was performed by loading Jurkat cell (40µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a SOCS6 antibody (Product # PA5-50263) at a 1/600 dilution for 1 second, followed by a secondary antibody at 1/8000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of SOCS6 in paraffin-embedded Human colon cancer tissue. Samples were probed using a SOCS6 polyclonal antibody (Product # PA5-50263) at a dilution of 1/35.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of SOCS6 in paraffin-embedded Human liver cancer tissue using SOCS6 Polyclonal Antibody (Product # PA5-50263) at a 1:50 dilution. (Original magnification: x200).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: EV2 Figure MiR-342-3p directly targets SOCS6 to regulate anti-Mtb immunity A Relative expressions of miR-342-2p target genes were analyzed by qRT-PCR. Data are shown as the mean +- SEM of n = 3 biological replicates. B Cell death mechanisms of RAW264.7 cells transfected with siRNA, followed by Mtb infection for 36 h. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. C, D MiR-342-3p mimic or inhibitor was transfected to RAW264.7 macrophages. After 48 h, cells were collected for qRT-PCR (C, data are shown as the mean +- SEM of n = 3 biological replicates) and Western blotting (D, representative blots from n = 3 biological replicates are shown) to detect the relative levels of SOCS6. E C3H BMDMs were transfected with miR-342-3p mimic or SOCS6-overexpressing lentivirus, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. F B6 BMDMs were transfected with miR-342-3p inhibitor or Socs6 siRNA, followed by Mtb infection. Cell death mechanisms were analyzed, respectively. Representative data (from n = 3 biological replicates) are shown as the mean +- SEM of technical replicates. G, H SOCS6-overexpressing vector (G) or Socs6 siRNA (H) was mixed with polyethylenimine to form a complex, which was used to infect mice by tail vein injection ( N /P ratio=8). Lungs were collected for transfection effic

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3 STAT3 del reduces in vitro growth, survival and migration of colon cancer cells. ( A ) CCK8 assay results show the proliferation rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . ( B ) TUNEL assay results show the apoptotic rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . ( C ) Wound healing assay results show the migration rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . * denotes p

PA5-50263

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