sc-615

antibody from Santa Cruz Biotechnology

Targeting: PMS1 MLH2, PMSL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-615

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-615, RRID:AB_632253

Product name

Anti-PMS1

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Clinicopathological characteristics of colorectal cancer with family history: an evaluation of family history as a predictive factor for microsatellite instability.

Park IJ, Kim HC, Yoon YS, Yu CS, Jang SJ, Kim JC
Journal of Korean medical science 2007 Sep;22 Suppl:S91-7

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BRAF-V600E is not involved in the colorectal tumorigenesis of HNPCC in patients with functional MLH1 and MSH2 genes

Enric Domingo, Renée C Niessen, Carla Oliveira, Pia Alhopuro, Catia Moutinho, Eloi Espín, Manel Armengol, Rolf H Sijmons, Jan H Kleibeuker, Raquel Seruca, Lauri A Aaltonen, Kohzoh Imai, Hiroyuki Yamamoto, Simó Schwartz, Robert M W Hofstra
Oncogene 2005 Mar;24(24):3995-3998

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 106

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.