sc-618

antibody from Santa Cruz Biotechnology

Targeting: PMS2 H_DJ0042M02.9, HNPCC4, MLH4, PMSL2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-618

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-618, RRID:AB_2167281

Product name

Anti-PMS2

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Clinicopathological characteristics of colorectal cancer with family history: an evaluation of family history as a predictive factor for microsatellite instability.

Park IJ, Kim HC, Yoon YS, Yu CS, Jang SJ, Kim JC
Journal of Korean medical science 2007 Sep;22 Suppl:S91-7

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Androgen receptor mutation (T877A) promotes prostate cancer cell growth and cell survival

C Sun, Y Shi, L L Xu, C Nageswararao, L D Davis, T Segawa, A Dobi, D G McLeod, S Srivastava
Oncogene 2006 Apr;25(28):3905-3913

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 63

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in purkinje cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.