DPABH-06661

antibody from Creative Diagnostics

Targeting: PMS2 H_DJ0042M02.9, HNPCC4, MLH4, PMSL2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

DPABH-06661

Provider

Creative Diagnostics

Proper citation

Creative Diagnostics Cat#DPABH-06661, RRID:AB_2521744

Product name

Rabbit Anti-Human PMS2 Polyclonal Antibody

Antibody type

Polyclonal

Description

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Concentration

Lot specific

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.