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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Product number
- MAB2089 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2089, RRID:AB_1711421
- Product name
- PMS2 monoclonal antibody, clone 349.29.5.2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant PMS2.
- Isotype
- IgG
- Antibody clone number
- 349.29.5.2
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references ATM-mediated stabilization of hMutL DNA mismatch repair proteins augments p53 activation during DNA damage.
Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation.
Microsatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer.
Hereditary nonpolyposis colorectal cancer and related conditions.
Luo Y, Lin FT, Lin WC
Molecular and cellular biology 2004 Jul;24(14):6430-44
Molecular and cellular biology 2004 Jul;24(14):6430-44
Mismatch repair gene PMS2: disease-causing germline mutations are frequent in patients whose tumors stain negative for PMS2 protein, but paralogous genes obscure mutation detection and interpretation.
Nakagawa H, Lockman JC, Frankel WL, Hampel H, Steenblock K, Burgart LJ, Thibodeau SN, de la Chapelle A
Cancer research 2004 Jul 15;64(14):4721-7
Cancer research 2004 Jul 15;64(14):4721-7
Microsatellite instability, immunohistochemistry, and additional PMS2 staining in suspected hereditary nonpolyposis colorectal cancer.
de Jong AE, van Puijenbroek M, Hendriks Y, Tops C, Wijnen J, Ausems MG, Meijers-Heijboer H, Wagner A, van Os TA, Bröcker-Vriends AH, Vasen HF, Morreau H
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Feb 1;10(3):972-80
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Feb 1;10(3):972-80
Hereditary nonpolyposis colorectal cancer and related conditions.
Lucci-Cordisco E, Zito I, Gensini F, Genuardi M
American journal of medical genetics. Part A 2003 Nov 1;122A(4):325-34
American journal of medical genetics. Part A 2003 Nov 1;122A(4):325-34
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown using PMS2 monoclonal antibody, clone 349.29.5.2 (Cat # MAB2089) to detect human PMS2 protein present in H157 cell lysates.Approximately 5, 10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer.A single band of ~96 KDa corresponding to the expected molecular weight for human PMS2 protein.Personal communication Morphotek Inc.
