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Learn200-301-864
antibody from Rockland Immunochemicals, Inc.
Targeting: PMS2
H_DJ0042M02.9, HNPCC4, MLH4, PMSL2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-864 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-864, RRID:AB_828384
- Product name
- Anti-PMS2 (MOUSE) Monoclonal Antibody - 200-301-864
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot analysis is shown using Rockland's Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.
- Validation comment
- Western Blot
