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Learn22779-1-AP
antibody from Invitrogen Antibodies
Targeting: LIFR
CD118
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 22779-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIFR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NNVEVLETR SAFPKIEDTE IISPVAERPE DRSDAEPENH VVVSYCPPII EEEIPNPAAD EAGGTAQVIY IDVQSMYQPQ AKPEEEQEND PVGGAGYKPQ MHLPINSTVE DIAAEEDLDK TAGYRPQANV NTWNLVSPDS PRSIDSNSEI VSFGSPCSIN SRQFLIPPKD EDSPKSNGGG WSFTNFFQNK PND (906-1097 aa encoded by B C153096)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.41 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 22779-1-AP (LIFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 22779-1-AP (LIFR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney slide using 22779-1-AP (LIFR Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 22779-1-AP ( LIFR Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with .2ug LIFR antibody (22779-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.
