600-401-281

antibody from Rockland Immunochemicals, Inc.

Targeting: MAPKAPK2 MK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-281

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-281, RRID:AB_217916

Product name

Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Affinity purified anti-MAPKAP Kinase 2 polyclonal antibody detects MK2 in unstimulated human HeLa whole cell lysate by western blot. Polyclonal rabbit-anti-MAPKAP Kinase 2 used at a 1:2000 dilution to detect 20 ug of whole cell lysate containing MK2. This antibody detects a single 44 kDa protein as indicated in crude extracts prepared from either unstimulated or TNFa stimulated human HeLa cell lysates. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Validation comment

Western Blot