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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-81501 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGF10 in Lane A: A549 Whole Cell Lysate (30 µg), Lane B: MCF7 Whole Cell Lysate (30 µg), Lane C: NCI-H460 Whole Cell Lysate (30 µg), Lane D: Mouse lung tissue Lysate (30 µg). Samples were probed using a FGF10 Polyclonal Antibody (Product # PA5-81501) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:23 kDa. Observed band size:42 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FGF10 Polyclonal Antibody (Product # PA5-81501) at 1:500 dilution. Lane A: A549 Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: NCI-H460 Whole Cell Lysate, Lane D: Mouse lung tissue lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 42 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human FGF10 in human prostatic carcinoma with FGF10 Polyclonal Antibody (Product # PA5-81501, 1:1,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FGF10 Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate, Lane B: 0.5 mg MCF-7 Whole Cell Lysate 4 µL with FGF10 Polyclonal Antibody (Product # PA5-81501) and 15 µL of 50 % Protein G agarose. Primary antibody: FGF10 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 42 kDa.
