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- Antigen structure
- References [1]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-26114 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PIM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels.
Morishita D, Katayama R, Sekimizu K, Tsuruo T, Fujita N
Cancer research 2008 Jul 1;68(13):5076-85
Cancer research 2008 Jul 1;68(13):5076-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIM2 Antibody [NBP2-26114] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag probed with NBP2-26114 (0.1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP2-26114 (0.1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114] - 5ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
