PAB29346

antibody from Abnova Corporation

Targeting: PIM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29346

Provider

Abnova Corporation

Product name

PIM2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human PIM2.

Antigen sequence

SGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPA
TVWSLGILLYDMVCGDIPFERDQEILEAELHFPAH
VSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTP
AEDVPLNPSKGGPAPLAWSL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line EFO-21 Lane 3: Human cell line A-431 Lane 4: Human liver tissue with PIM2 polyclonal antibody (Cat # PAB29346) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with PIM2 polyclonal antibody (Cat # PAB29346) at 1-4 ug/mL concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with PIM2 polyclonal antibody (Cat # PAB29346) shows cytoplasmic positivity in cells of seminiferus ducts at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)