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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA501061 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501061, RRID:AB_11126154
- Product name
- PIM2 mouse monoclonal antibody, clone OTI5B5 (formerly 5B5)
- Antibody type
- Monoclonal
- Description
- PIM2 mouse monoclonal antibody, clone OTI5B5 (formerly 5B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PIM2
- Isotype
- IgG
- Antibody clone number
- OTI5B5
- Vial size
- 100 µl
- Concentration
- 0.89 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PIM2 mouse monoclonal antibody (TA501061) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2(RC201933).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501061)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501061)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501061)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201933 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIM2 antibody(TA501061), and then analyzed by flow cytometry.
- Validation comment
- FC
