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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 701283 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HGF Recombinant Rabbit Monoclonal Antibody (7H 6L1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
- Antibody clone number
- 7H 6L1
- Concentration
- 0.5 mg/mL
Submitted references Ultra Sensitivity Silicon-Based Photonic Crystal Microcavity Biosensors for Plasma Protein Detection in Patients with Pancreatic Cancer.
Cabozantinib and Tivantinib, but Not INC280, Induce Antiproliferative and Antimigratory Effects in Human Neuroendocrine Tumor Cells in vitro: Evidence for 'Off-Target' Effects Not Mediated by c-Met Inhibition.
Yang CJ, Yan H, Tang N, Zou Y, Al-Hadeethi Y, Xu X, Dalir H, Chen RT
Micromachines 2020 Mar 9;11(3)
Micromachines 2020 Mar 9;11(3)
Cabozantinib and Tivantinib, but Not INC280, Induce Antiproliferative and Antimigratory Effects in Human Neuroendocrine Tumor Cells in vitro: Evidence for 'Off-Target' Effects Not Mediated by c-Met Inhibition.
Reuther C, Heinzle V, Spampatti M, Vlotides G, de Toni E, Spöttl G, Maurer J, Nölting S, Göke B, Auernhammer CJ
Neuroendocrinology 2016;103(3-4):383-401
Neuroendocrinology 2016;103(3-4):383-401
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HGF in whole cell extracts from HepG2 cells using a HGF recombinant rabbit monoclonal antibody (Product # 701283) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~69kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HGF in HepG2 cells using a HGF recombinant rabbit monoclonal antibody (Product # 701283) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of HGF.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HGF in HepG2 cells using a HGF recombinant rabbit monoclonal antibody (Product # 701283). Cells were fixed and permeabilized using FIX & PERM (Product # GAS004) reagent, and detection was performed using an Alexa Fluor 488 goat anti-rabbit IgG (right peak) compared to a control without primary antibody (left peak).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
