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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51874PU-N - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal anti-GNAS (C-term) Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal anti-GNAS (C-term) Antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GNAS3
- Isotype
- IgG
- Vial size
- 0.4ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of GNAS (arrow) using GNAS Antibody (C-term) Cat.-No AP51874PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of GNAS Antibody (C-term) Cat.-NoAP51874PU-Nin 293, NCI-H292 cell line lysates (35ug/lane). This demonstrates the GNAS antibody detected the GNAS protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GNAS Antibody (C-term) Cat.-NoAP51874PU-Nwith 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with GNAS Antibody (C-term) Cat.-NoAP51874PU-Nfollowed by peroxidase conjugation of the secondary antibody and DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using GNAS Antibody (C-term) Cat.-NoAP51874PU-N(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
