Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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Validation data
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- Product number
- GTX15750 - Provider product page
- Provider
- GeneTex
- Product name
- Interferon gamma antibody [B133.5]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Purified human CD4+ T cells were left non-stimulated (Lane 1), stimulated with anti-CD3 (Lane 2), or stimulated with anti-CD3 and anti-CD28 (Lane 3) for three days. IFN gamma production (pg/ml) was determined by ELISA by coating IFN gamma antibody [2G1] (1:1000), blocking with 1% BSA in PBS, and detection with IFN gamma antibody [B133.5] (1:1000), followed by peroxidase-conjugated avidin and TMB substrate.
- Validation comment
- ELISA