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Learnsc-8349
antibody from Santa Cruz Biotechnology
Targeting: RAD51
BRCC5, FANCR, HsRad51, HsT16930, RAD51A, RECA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8349 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8349, RRID:AB_2253533
- Product name
- Anti-RAD51
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Aurora kinase A promotes ovarian tumorigenesis through dysregulation of the cell cycle and suppression of BRCA2.
Activity of ribonucleotide reductase helps determine how cells repair DNA double strand breaks.
Thoc1 deficiency compromises gene expression necessary for normal testis development in the mouse.
Identification of SMARCAL1 as a component of the DNA damage response.
A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
Apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45
Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks.
Viral transport of DNA damage that mimics a stalled replication fork.
Yang G, Chang B, Yang F, Guo X, Cai KQ, Xiao XS, Wang H, Sen S, Hung MC, Mills GB, Chang S, Multani AS, Mercado-Uribe I, Liu J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jun 15;16(12):3171-81
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jun 15;16(12):3171-81
Activity of ribonucleotide reductase helps determine how cells repair DNA double strand breaks.
Burkhalter MD, Roberts SA, Havener JM, Ramsden DA
DNA repair 2009 Nov 2;8(11):1258-63
DNA repair 2009 Nov 2;8(11):1258-63
Thoc1 deficiency compromises gene expression necessary for normal testis development in the mouse.
Wang X, Chinnam M, Wang J, Wang Y, Zhang X, Marcon E, Moens P, Goodrich DW
Molecular and cellular biology 2009 May;29(10):2794-803
Molecular and cellular biology 2009 May;29(10):2794-803
Identification of SMARCAL1 as a component of the DNA damage response.
Postow L, Woo EM, Chait BT, Funabiki H
The Journal of biological chemistry 2009 Dec 18;284(51):35951-61
The Journal of biological chemistry 2009 Dec 18;284(51):35951-61
A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor.
Turner NC, Lord CJ, Iorns E, Brough R, Swift S, Elliott R, Rayter S, Tutt AN, Ashworth A
The EMBO journal 2008 May 7;27(9):1368-77
The EMBO journal 2008 May 7;27(9):1368-77
Apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45
R Rodriguez, M E Gagou, M Meuth
Cell Death and Differentiation 2008 Feb;15(5):889-898
Cell Death and Differentiation 2008 Feb;15(5):889-898
Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks.
Potts PR, Porteus MH, Yu H
The EMBO journal 2006 Jul 26;25(14):3377-88
The EMBO journal 2006 Jul 26;25(14):3377-88
Viral transport of DNA damage that mimics a stalled replication fork.
Jurvansuu J, Raj K, Stasiak A, Beard P
Journal of virology 2005 Jan;79(1):569-80
Journal of virology 2005 Jan;79(1):569-80
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 37
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells of seminiferus ducts.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
