- MA1-23271 - Invitrogen Antibodies RAD51 antibody

van Sluis M, McStay B
Genes & development 2015 Jun 1;29(11):1151-63

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAD51 in Hela (lane 1) and T24 (lane 2) cell lysates. Samples were probed with a RAD51 monoclonal antibody (Product # MA1-23271).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouset IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500 and a HRP-conjugated anti-mouset IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RAD51 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RAD51 was achieved by transfecting HeLa cells with RAD51 specific siRNAs (Silencer® select Product # s11734, s11735). Western blot analysis (Fig. a) was performed using whole cell extracts from the RAD51 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RAD51.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of NIH/3T3 (Lane 1), HeLa (Lane 2), RAW 264.7 (Lane 3), Neuro-2A (Lane 4), Jurkat (Lane 5), Raji (Lane 6) and PANC-1 (Lane 7). The blot was probed with Anti-RAD51 Monoclonal Antibody (Product # MA1-23271, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to RAD51 was observed in all the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorecent staning of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody (Product # MA1-23271). Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1,000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: RAD51 Monoclonal Antibody (14B4) detects Rad51 protein at nuclear foci by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad51 stained by RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunoprecipitation of HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40). Samples were immunoprecipitated using a monoclonal RAD51 antibody (Product # MA1-23271) followed by Western blot using RAD51 polyclonal antibody (Product # PA5-27195). Whole cell extract was used as input.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. RAD51 Monoclonal Antibody (14B4) (Product # MA1-23271) dilution: 1:200. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Supportive validation

MA1-23271